Counting absolute numbers of molecules using unique molecular identifiers Event as iCalendar

05 February 2019

1 - 2pm

Venue: Mac 1 Biology Building

Location: 5 Symonds St

Host: SBS

Contact info: Professor Ant Poole

Contact email: a.poole@auckland.ac.nz

Professor Arndt von Haeseler
Professor Arndt von Haeseler

Speaker: Professor Arndt von Haeseler, Scientific Director at Max F. Perutz Laboratories, Vienna Biocenter / Professor of Bioinformatics, University of Vienna/Medical University of Vienna.



Counting DNA or RNA molecules using next-generation sequencing (NGS) suffers from amplification biases. Counting unique molecular identifiers (UMIs) instead of reads is still prone to over-estimation due to amplification and sequencing artifacts and under-estimation due to lost molecules. We present an algorithm that corrects for these errors, based on a mechanistic model of the PCR and sequencing process whose parameter have an immediate physical interpretation and are easily estimated. We demonstrate that our algorithm outputs essentially unbiased counts with substantially improved accuracy.

For more information please contact Professor Ant Poole.